Chloroquine resazurin assay results

Discussion in 'International Pharmacy' started by v_roger, 19-Mar-2020.

  1. madausmarta Well-Known Member

    Chloroquine resazurin assay results


    Current treatment options are, at best, palliative. The objectives of this study were to evaluate a panel of nineteen candidate compounds for antiviral activity against FCo V to determine viable candidates for therapy.

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    Dec 20, 2001 Methods and Results Resazurin was incubated with a range of organic chemicals that were used as substrates in an MPN assay. Only 1,2‐dichlorobenzene, 2,4‐ D, glycol sulphite and sulphinol reacted to generate false positives. Resazurin was also used to estimate micro‐organisms in a series of bioremediation studies. Results Alamar Blue and resazurin are the same compound. Confirmation of the identity of Alamar Blue as resazurin was obtained via high‐field 1 H NMR spectroscopy and tandem mass spectrometry of material isolated from the Alamar Blue reagent by solid‐phase extraction as described in Materials and methods. The spectra obtained for Alamar. Thaw out Resazurin solution if kept frozen and warm it to 37°C to ensure all components are completely in solution. Plate cells in 96-well plate black plate with clear bottom. Typical seed cell number is 0.02–2 x 10 5 cells/well depending on cell growth rate.

    Plaque reduction and virus yield reduction assays were performed to confirm antiviral effects of candidate compounds identified during screening, and the possible antiviral mechanisms of action of these compounds were investigated using virucidal suspension assays and CPE inhibition and IFA-based time of addition assays. A resazurin-based cytopathic effect inhibition assay, which detects viable cells through their reduction of the substrate resazurin to fluorescent resorufin, was developed for screening compounds for antiviral efficacy against FCo V.

    Chloroquine resazurin assay results

    CST - Resazurin Cell Viability Kit, Investigation of the Alamar Blue resazurin fluorescent dye.

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  3. Resazurin assay. The resazurin assay also known as Alamar Blue assay offers a simple, rapid, and sensitive measurement for the viability of mammalian cells and bacteria. Living cells are metabolically active and are able to reduce via mitochondrial reductase, the nonfluorescent dye resazurin to the strongly-fluorescent dye resorufin Fig.

    • Resazurin Assay - an overview ScienceDirect Topics.
    • Resazurin Cell Viability Kit Protocol CST.
    • Resazurin Sigma-Aldrich.

    Resazurin undergoes a redox reduction to resorufin, a pink, fluorescent chemical O’Brien et al. 2000. The redox reaction in resazurin-based assays provides information about the intercellular processes and metabolic activity. Under typical assay conditions, the active component, resazurin, is reduced to resorufin Candeias et al. 1998. This chemical change, changes the physical properties of the medium allowing detection via fluorescence or spectrophotometry. Resazurin 7-Hydroxy-3 H -phenoxazin-3-one 10-oxide is a blue dye, itself weakly fluorescent until it is irreversibly reduced to the pink colored and highly red fluorescent resorufin. It is used as an oxidation-reduction indicator in cell viability assays for both aerobic and anaerobic respiration. Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This kit performs at least as well as other commercial resazurin-based cell proliferation assay kits such as alamarBlue® or CellTiter-Blue®.

     
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